Yes, absolutely! If you sell globally and you have multiple locations, naturally when an order comes into your eCommerce store you would want to select the closest or most convenient location to ship your orders from.
When your sales orders are pushed into TradeGecko they come in as Finalized orders. At this stage you can edit the Ship From location.
Go to Sales Orders and select the order that you need to update.
Click on the "Edit Order" button.
In the edit page, select a new location from the "Ship From" field.
Select your new Ship From Location and click on "Save".
You can also ship your Sales Order line items from different locations, find out how.